CH 150: Introduction to Biochemistry

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Protein Purification

        In this experiment, you will be selecting one colony of transformed bacteria from the previous experiment and growing a larger colony of bacteria.  You will then rupture the bacteria and use column chromatography with a hydrophobic resin to isolate the green fluorescent protein from the total cell lysate. 

          We will be using a kit developed by Bio-Rad Laboratories as a part of their Biotechnology Explorer Curriculum. Follow the link to the Student Manual. Print a copy of the manual to follow in the lab.  Your completed copy of the manual will serve as your lab report.